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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
02/01/2008 |
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Data da última atualização: |
08/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. |
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Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP; FLÁVIO VIEIRA MEIRELLES, USP; WALT YAMAZAKI, UNESP; MARCOS ROBERTO CHIARATTI, USP; SIMONE CRISTINA MEO NICIURA, CPPSE; FELIPE PERECIN, UNESP; LAWRENCE CHARLES SMITH, UNIVERSITÉ DE MONTRÉAL; JOAQUIM MANSANO GARCIA, UNESP. |
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Título: |
The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007. |
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DOI: |
10.1089/clo.2006.0082 |
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Idioma: |
Inglês |
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Conteúdo: |
The mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth. MenosThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either un... Mostrar Tudo |
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Palavras-Chave: |
Bovine embryos; Cell mtDNA; Embryonic. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02452naa a2200253 a 4500
001 1048156
005 2023-03-08
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1089/clo.2006.0082$2DOI
100 1 $aFERREIRA, C. R.
245 $aThe kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos.$h[electronic resource]
260 $c2007
520 $aThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth.
653 $aBovine embryos
653 $aCell mtDNA
653 $aEmbryonic
700 1 $aMEIRELLES, F. V.
700 1 $aYAMAZAKI, W.
700 1 $aCHIARATTI, M. R.
700 1 $aNICIURA, S. C. M.
700 1 $aPERECIN, F.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
773 $tCloning and Stem Cells.$gv. 9, n. 4, p. 618-629, dec. 2007.
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| 8. |  | SILVA, S. C. da. Clima. In: STONE, L. F.; MOREIRA, J. A. A.; RABELO, R. R.; BIAVA, M. (Ed.). Arroz: o produtor pergunta, a Embrapa responde. Brasília: Embrapa Informação Tecnológica, 2001. p. 13-21. (Coleção 500 perguntas 500 respostas).| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 9. | | SILVA, S. C. da. Clima. 2.ed.rev.ampl. In: SANTIAGO, C. M.; BRESEGHELLO, H. C. de P.; FERREIRA, C. M. (Ed.). Arroz: o produtor pergunta, a Embrapa responde. 2. ed. rev. e ampl. Brasília, DF: Embrapa, 2013. p. 15-23. (Coleção 500 perguntas, 500 respostas).| Tipo: Capítulo em Livro Técnico-Científico |
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